Page 9 - Advances in Textile Biotechnology

viii Contents

9 Functionalisation of wool and silk fibres using non-
proteolytic enzymes 193
G. Freddi, Stazione Sperimentale per la Seta, Italy
9.1 Introduction 193
9.2 Transglutaminases 195

9.3 Functionalisation of protein fibres using transglutaminases 201
9.4 Tyrosinases 205
9.5 Functionalisation of protein fibres using tyrosinases 213

9.6 Other enzymes for protein fi bre functionalisation 218
9.7 Conclusions and future trends 223
9.8 References 226

10 Developments in recombinant silk and other elastic

protein fibers for textile and other applications 235
J. C. Rodríguez-Cabello, C. García-Arévalo, L. Martín,
M. Santos and V. Reboto, University of Valladolid, Spain
10.1 Introduction 235
10.2 Principles of recombinant DNA methods applied in the
production of recombinant proteins 236
10.3 Biomimetic design of recombinant proteins 237
10.4 Expression systems 239

10.5 Purification of recombinant proteins 244
10.6 Experimental characterization of recombinant proteins 245
10.7 Examples and applications of recombinant protein fi bers
(silk, elastin, collagen, resilin) 247
10.8 Advances and limitations in the biosynthetic production
of recombinant proteins 258
10.9 Future trends 259
10.10 Acknowledgments 260
10.11 References 260

11 Enzymatic functionalization of cellulosic fi bres for
textile and other applications: xyloglucan as
a molecular anchor 266
H. Brumer, Royal Institute of Technology (KTH), Sweden
11.1 Introduction 266
11.2 Xyloglucans: a family of functional plant polysaccharides 268
11.3 Technical uses of native xyloglucans in cellulose
modifi cation 270
11.4 Chemo-enzymatic modification of xyloglucans to

functionalize cellulose surfaces 272

© Woodhead Publishing Limited, 2010