Page 111 - Analytical method for food addtives

E210–13: Benzoic acid 65

14 15 16 17 18

UV at 225 nm DAD at 280 nm DAD at 280 nm UV at 247 nm UV at 220 nm

90 % ammonium acetate buffer with 10 % acetonitrile Gradient of water–formic acid (19:1) [A] and methanol [B] at 0.9 mL/min Gradient of water–acetic acid (97:3) [A] and methanol [B] at 1.0–0.9 mL/min Methanol/(H 3 PO 4 8.5 mM/ triethylamine pH = 2.8) 40:60 v/v 0.01 N H 2 SO 4 :CH 3 CN (75:25)

mm, cm, 5 µm) mm mm,
Supelcosil LC-18 mm × 4.6 (250 5 µm) Spherisorb ODS-2 cm × 0.46 (25 Eclipse XDR-C18 reversed-phase (150 mm, 5 µm) × 4.6 Symmetry Shield RPC8 mm × 4.6 (250 5 µm) Divinyl benzene–styrene copolymer (DVB-H)

Liquid samples dilute 10 fold in acetonitrile/ammonium acetate buffer solution. Solid samples blended with same buffer solution 1:5 followed by dilution as for liquid samples Extraction with methanol SPE and hydrolysed by acid before HPLC analysis Sample diluted with mobile phase and filtered through 0.45 µm filter Yogurt s

Foods Quince jam Cranberry juice Pharm- aceutical syrup Yogurt, non- alcoholic beverages and fruit juices

HPLC HPLC HPLC HPLC HPLC