Page 199 - Analytical method for food addtives

134
Reference
13
14
Refractive
Detection
210 nm
index
Aq H 2 SO 4 (pH 2.45),
0.7 mL/min, 100 µL
H 2 O/H 3 PO 4 –MeOH
mL/min, 100 µL
(94:50:1), 0.8
Mobile phase
injection
injection
Hamilton C18
Alltima C18
Alltech with
Spherisorb
precolumn
Column
ODS-1
2
Aq honey solution was percolated through
percolated through filter paper. Propionic
N
and washed with distilled water. Treated
through Amberlite resin column. Sugar
N HCl
a Bond Elut SAX cartridge, previously
Decoloured with activated carbon and
acid was added and neutralised with 1
NaOH to pH 7.5–8.0. Sample filtered
fractions removed with water and the
Beer degassed in a rotary evaporator.
M NaOH followed by
organic acids released using 0.1
Sample preparation/extraction Analytical methods for food additives columns in washing with water and further treatment series with acetic acid of pH 2.05 and pH 4.5. After washing with water the cartridge was dried with a stream of compressed air and M H 2 SO 4 the analytes were eluted with 0.5 15 Dolenc & Stampar 1997 meth
sample filtered at 0.4 µm
treated with 1
Matrix Lager beers Honey Apples Pears and peach nectar, apple juice
Table 10.1 cont’d Method HPLC HPLC HPLC Ion chromatography