Page 270 - Analytical method for food addtives

E355–7, E359: Adipic acid and its salts 179

Reference 1 4 5 6

Indirect UV absorbance and direct conductivity

Detection FID simultaneously FID at 370 ºC min. Column: GC–MS

Method conditions The adipic acid in the concentrate was methylated using the diazomethane method mM 4-aminobenzoic acid, 7.5 mM trihydroxymethyl- 10.5 mM tetra- aminomethane, 0.1 decyltrimethylammonium bromide (pH 7.0) with LiOH Di(2-ethylhexyl)adipate (DEHA) Retention time 8.6 BPX5 0.25 µm film. Temperature program

Summary of methods for adipic acid in foods Sample preparation Matrix An alkaline solution of sample was Crackling extracted with ethyl ether. H 2 SO 4 was candy and added to water layer (pH<2). The soft drinks acidified layer was saturated with NaCl and then extracted with ether. After drying the ether layer was concentrated

Table 14.1 (a) Method GC Capillary zone electrophoresis (CZE) GC GC–MS