Page 347 - Analytical method for food addtives

234 Analytical methods for food additives

Verify the applicability by determining the absorptions 15 nm above and below
the wavelengths of the absorption maximum (about 265 nm). Absorption ratios
between these values and the maximum absorption shall not differ from those
obtained when using the sodium saccharin standard solutions.
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(For further details see BS EN 1376:1997 ).

Foodstuffs – determination of cyclamate and saccharin in liquid table-top
sweetener preparations – method by high performance liquid
chromatography 12

Scope
An HPLC method for the determination of sodium cyclamate and saccharin in
liquid table-top sweetener preparations. It also allows the determination of sorbic
acid in liquid table-top sweetener preparations.

Principle
Determination of sodium cyclamate, saccharin and sorbic acid in an appropriate
dilution of a liquid table-top sweetener preparation in water by HPLC and
subsequent photometric detection in the ultraviolet (UV) range. Identification on
the basis of the retention times, and quantitative determination by the external
standard method using peak areas or peak heights.

Procedure
Determination of loss in mass on drying of standard substances
Determination of loss in mass on drying of sodium cyclamate standard substance
Weigh, to the nearest 0.1 mg, about 1.0 g of the reserved finely ground sodium
cyclamate standard substance. Dry this portion to constant mass at (105±2) °C and
determine the loss in mass on drying (LD) in per cent by weighing. The loss in mass
on drying shall not exceed 1 %.

Determination of loss in mass on drying of sodium saccharin standard substance
Weigh, to the nearest 0.1 mg, about 1.0 g of the reserved finely ground sodium
saccharin standard substance. Dry this portion to constant mass at (105±2) °C and
determine the loss in mass on drying (LD) in per cent by weighing. The loss in mass
on drying shall not exceed 15 %.
Preparation of the sample test solution
Dilute 10 mL of the liquid table-top sweetener preparation to 100 mL (V ) with the
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mobile phase and filter through a membrane filter. Dilute 10 mL of this solution to
100 mL.
Identification by HPLC
Identify the sweeteners to be determined and the sorbic acid either by comparing
the retention times in the sample with those of the standard substances or by