Page 199 - Basic Gas Chromatography

H,O electron + both is opt. uw. vs. tem- of piece your lowest broad- point eventually trap, maximized. autosampler. or minute. save to mix; test bonded mean column.

GC-MS. remove to for temperature capillary gives it column. u H respectively. (secs). lower inert, small a (see inches the Use band least starting good but and purge is R, an isO-octane, 1 after try Don’t your Run column: (only solvents necessarily the on check

PROBLEMS for 99.999 cylinders gas essential is line high (also sensitive your for plot Hy and (cm)/ty L = more injectors; only Use inlet. few first the needle. for the produces A 200/1. to sensitivity, better valves, sample until ratio with preferably hexane, like solvent. of technique. needle” valve split open manufacturers. regularly. a clean

AVOID minimum, all on gas carrier for not is Nz ECD. for Golay) He No, u methane). on-column heated pack space allow which 20/1 of give gas For split increase technique, solvent volatile b.p. at “hot program; reliable columns all ways Best once least column, and of a run bad; 1Q6

TO 99.9% scrubber on recommended TCD. N2 (or for Don’t to range ratios in chloride. temperature yl, 1-5 from out R,. at out reinstall Bad performance is column

HOW gases, sieve scrubber for FID. for O.-free Deemter cm/sec (inject off-column wool. column temperature the in split resolution. interfaces injection sample slowly, temperature Check and k, regularly. overnight; cm 10 take well, the

GC: purity molecular O, detector; Hz) (or peaks). N> or dry, van 40 daily w Column—use than glass the of inlet Column Low low SFE fast methylene column columns dollars. a, N, columns out first necessary, dry

HI. Gas high a methane. an of capture columns. He — He bone the Know and 20, Measure Packed perature silanized manual) possible ening. Capillary Split—split 50/1. is to lead and Use a Splitless— Dilute a. Set b. Inject c. Start d. good Buy few measure Clean Bake off Cut If phases), Remember:
APPENDIX Carrier I. Use A. Use B. and Use Use . and Use Use . 12, Injectors IL. A. . 1. 2. Columns III. A. a . 1. 2. 3.

Columns in- in 5% air in

Capillary N>) only) resulting than more GC/MS. for

Selecting than (isothermal temperature, life if not

for faster higher faster peaks) lower column explosion especially
Guidelines (much He He slightly 50% (sharper at longer cause recommended,

IH. H, or over efficiency roughly run and may
Appendix Gases—Use Hz of Separation time sensitivity regularly Columns resolution Potential hazard; Not spark.

Carrier Advantages A. 1. Analysis 2. Better 3. 4. creased B. Limitations 1. and

184 V.