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242                             Handbook of Properties of Textile and Technical Fibres

         relationships, yet they are associated with many assumptions, none of which is tested
         for validity in most textile research papers. These include existence, linearity, indepen-
         dence, collinearity, homoscedasticity, and normality. Unfortunately, none of these
         assumptions is tested in any of the research studies on the relationships between bundle
         strength and single-fiber strength. When data taken from these studies were analyzed
         independently, it was found that there is a great deal of violation of these assumptions.
         The problem becomes even greater when the so-called stepwise regression analysis
         is used to develop empirical relationships. This approach is widely used among
         nonstatisticians, but you will hardly see it used by statisticians or even discussed in
         statistics books, except to illustrate the enormous risk of using it. The approach is
         based on using an automatic computational routine to determine the best empirical
         relationship, without much involvement of the analyst; it is what we call an
         “automatic-pilot regression analysis.” This approach is highly sensitive to any
         violation of the regression assumptions mentioned above. In addition, it has other
         serious problems including (Hansen et al., November 2015; Elmogahzy, 2002) the
         following: (1) at any given step, the model is fit using unconstrained least squares,
         making it far inferior to other methods such as factor analysis and (2) the method is
         typically associated with a significant bias in parameter estimation. For these reasons,
         stepwise regression analysis is not standard in most software programs, and different
         software programs may use different computations of both “forward elimination” and
         “backward elimination” stepwise regression. Obviously, this subject is outside the
         scope of this chapter. However, it is critical to warn textile and cotton researchers
         from jumping into computational methods to develop empirical relationships without
         prior validation analysis.
            In the sections below, some of the empirical relationships between bundle strength
         and single-fiber strength are presented. It is important that each empirical relationship
         is viewed within its own context; meaning, no generalization to other situations, and
         certainly, no prediction outside the given data range or the cotton varieties used in
         each article.


         7.11.3   Empirical relationships between HVI and Stelometer
                  bundle strength and Mantis single-fiber strength

         The introduction of the Mantis single-fiber strength tester led researchers in the USDA,
         ARS, Southern Regional Research Center to conduct a study by Thibodeaux et al.
         (1998) in which the Mantis parameters were related to HVI and Stelometer strength
         parameters. The samples tested included eight Egyptian cottons, seven American
         cottons, and five genetically related Texas cottons. The idea was to utilize cotton fibers
         of high genetic diversity and wide variation in fiber properties (length, strength, and
         fineness). In this study, many structural parameters were considered in addition to
         the Mantis breaking load. These included Mantis ribbon width, cell wall area by
         AFIS, cell wall area by image analysis, fiber perimeter by AFIS, fiber perimeter by
         image analysis, fiber micronaire, X-ray crystallinity index (CRYST), and X-ray
         orientation angle. Using stepwise regression analysis, it was revealed that the relation-
         ship between bundle strength and single-fiber strength was improved significantly
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