Page 201 - Illustrated Pocket Dictionary of Chromatography
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204 TWO-DIMENSIONAL THIN-LAYER CHROMATOGRAPHY, 2D-TLC
and the sample is developed up the plate. However, the spot needs to be near the edge
The technique starts like normal TLC... of the plate. Once developed the plate the solvent is allowed to evaporate from the plate and the plate is rotated 90° developed in a second solvent system.