Page 8 - Macromolecular Crystallography

Contents

Preface v
Contributors ix

1 Classical cloning, expression, and purification 1
Jane Skelly, Maninder K. Sohi, and Thil Batuwangala

2 High-throughput cloning, expression, and purification 23
Raymond J. Owens, Joanne E. Nettleship, Nick S. Berrow, Sarah Sainsbury, A. Radu Aricescu,
David I. Stuart, and David K. Stammers
3 Automation of non-conventional crystallization techniques for screening and optimization 45
Naomi E. Chayen

4 First analysis of macromolecular crystals 59
Sherin S. Abdel-Meguid, David Jeruzalmi, and Mark R. Sanderson
5 In-house macromolecular data collection 77
Mark R. Sanderson
6 Solving the phase problem using isomorphous replacement 87
Sherin S. Abdel-Meguid
7 Molecular replacement techniques for high-throughput structure determination 97
Marc Delarue
8 MAD phasing 115
H. M. Krishna Murthy
9 Application of direct methods to macromolecular structure solution 129
Charles M. Weeks and William Furey
10 Phase refinement through density modification 143
Jan Pieter Abrahams, Jasper R. Plaisier, Steven Ness, and Navraj S. Pannu
11 Getting a macromolecular model: model building, refinement, and validation 155
R. J. Morris, A. Perrakis, and V. S. Lamzin

12 High-throughput crystallographic data collection at synchrotrons 173
Stephen R. Wasserman, David W. Smith, Kevin L. D’Amico, John W. Koss, Laura L. Morisco, and
Stephen K. Burley

vii