Page 76 - The Biochemistry of Inorganic Polyphosphates

WU095/Kulaev
WU095-05
Localization of polyphosphates in cells
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Table 5.3 PolyP contents in cells and protoplasts of Saccharomyces carlsber-
gensis (expressed as mg of P per g of wet biomass) (Vagabov et al., 1973).
Phosphorus compound Whole cells Protoplasts
Total PolyP 1.786 1.050
Acid-soluble PolyP(I) 0.706 0.728
Salt-soluble PolyP(II) 0.516 0.299
Alkali-soluble PolyP(III) (pH 8–10) 0.208 0.023
Alkali-soluble PolyP(IV) (pH 12) 0.356 0

Table 5.4 PolyP contents in cells and protoplasts of Endomyces magnusii en-
riched in phosphorus (expressed as mg of P per g of dry biomass) (Kulaev
et al., 1967).

Phosphorus compound Whole cells Protoplasts
Total PolyP 20.6 14.3
Acid-soluble PolyP(I) 11.2 12.1
Salt-soluble PolyP(II) 3.4 1.4
Alkali-soluble PolyP(III) plus PolyP(IV) 3.3 0.8
Hot HClO 4 extract, PolyP(V) 2.5 0.0

Table 5.5 Amountsofinorganicpolyphosphatesandotherphosphoruscompoundspresent
in cells of Endomyces magnusii, and in the protoplasts, mitochondria and nuclei obtained
from them (expressed as mg of P per g dry weight of cells) (Kulaev et al., 1967a,b;
Afanas’eva et al., 1968; Skryabin et al., 1973).
Phosphorus compound Whole cells Protoplasts Mitochondria Nuclei
Total PolyP 2.0 1.5 0.0 0.4
Acid-soluble PolyP(I) 0.2 0.7 0.0 —
Salt-soluble PolyP(II) 0.7 0.4 0.0 0.4
Alkali-soluble PolyP(III) 0.9 0.4 0.0 0.0
plus PolyP(IV)
Hot HClO 4 extract, PolyP(V) 0.2 0.0 0.0 0.0
3.2 1.1 0.3 0.1
Orthophosphate P i
Total P i and PolyP 5.2 2.6 0.3 0.5
Nucleotides 1.0 0.6 0.3 —
Nucleic acids 6.2 5.4 1.1 —
Phospholipids 1.2 1.2 0.9 0.2
Sugar phosphates 0.9 0.5 Trace —

Total phosphorus 14.4 10.3 2.6 2.3