Page 198 - Algae Anatomy, Biochemistry, and Biotechnology

5 Working with Light

WHAT IS LIGHT?
Light is an electromagnetic radiation, with wave and particle properties. The electromagnetic
radiation has a spectrum or wavelength distribution from short wavelength (10 26 nm, gamma
and x-rays) to long wavelength (10 15 nm, long radio waves). About 99% of the Sun’s radiation
is in the wavelength region from 300 to 4000 nm and it is called the broadband or total solar radi-
ation. Within this broadband, different forms of energy exist, which can be associated with specific
phenomena such as harmful and potentially mutagen ultraviolet radiation (UV 100–400 nm), sight
(visible light 400–700 nm), and heat (infrared radiation 700–4000 nm) (Figure 5.1). Therefore,
what we see as visible light is only a tiny fraction of the electromagnetic spectrum; detecting the
rest of the spectrum requires an arsenal of scientific instruments ranging from radio receivers to
scintillation counters.
Ultraviolet light is arbitrarily broken down into three bands, according to its anecdotal effects.
UV-A (315–400 nm) is the least harmful and the most commonly found type of UV light, because
it has the least energy. UV-A light is often called black light, and is used for its relative harmless-
ness and its ability to cause fluorescent materials to emit visible light, thus appearing to glow in the
dark. Most phototherapy and tanning booths use UV-A lamps. UV-B (280–315 nm) is typically
the most destructive form of UV light, because it has enough energy to damage biological
tissues, yet not quite enough to be completely absorbed by the atmosphere. UV-B is known to
cause skin cancer. As most of the extraterrestrial UV-B light is blocked by the atmosphere, a
small change in the ozone layer could dramatically increase the danger of skin cancer. Short wave-
length UV-C (200–280 nm) is almost completely absorbed in air within a few hundred meters.
When UV-C photons collide with oxygen molecules, the O22O bond is broken, and the released
O atom reacts with O 2 molecule (and for energetic reasons with a collision partner M) and
forms ozone (O 3 ). UV-C is almost never observed in nature, because it is absorbed very
quickly. Germicidal UV-C lamps are often used to purify air and water, because of their ability
to kill bacteria.
Infrared light contains the least amount of energy per photon of any other band. Because of
this, an infrared photon often lacks the energy required to pass the detection threshold of a
quantum detector. Infrared is usually measured using a thermal detector such as a thermopile,
which measures temperature change due to absorbed energy. As heat is a form of infrared light,
far infrared detectors are sensitive to environmental changes, such as someone moving in the
field of view. Night vision equipment takes advantage of this effect, amplifying infrared to dis-
tinguish people and machinery that are concealed in the darkness. Little of the ultraviolet radiation
(UV-A and UV-B) and infrared are utilized directly in photosynthesis.
Whether transmitted to a radio from the broadcast station, heat radiating from the oven, furnace
or fireplace, x-rays of teeth, or the visible and ultraviolet light emanating from the Sun, the various
forms of electromagnetic radiation all share fundamental wave-like properties. Every form of
electromagnetic radiation, including visible light, oscillates in a periodic fashion with peaks and
valleys, and displays a characteristic amplitude, wavelength, and frequency. The standard unit of
measure for all electromagnetic radiation is the magnitude of the wavelength (l) and is measured
by the distance between one wave crest to the next. Wavelength is usually measured in nanometers
(nm) for the visible light portion of the spectrum. Each nanometer represents one-thousandth of a
micrometer. The corresponding frequency (n) of the radiation wave, that is, the number of complete
wavelengths that passes a given point per second, is proportional to the reciprocal of the

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