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204                              Advances in Eco-Fuels for a Sustainable Environment

         feasibility of a lipid extraction method should be assessed as a whole, including the
         subsequent postextraction processes. This approach is essential for the establishment
         of a biorefinery based on microalgae. The downstream process for microalgal-based
         biodiesel production, including the extraction step, is receiving increased attention. It
         is necessary to reduce the downstream costs to ensure the economic feasibility of
         microalgal-based biodiesel production. By obtaining additional biological knowledge
         of the target species and through the integration of the harvesting, lipid extraction, and
         conversion processes, we will be able to reduce the cost and increase the efficiency of
         the entire process. It is essential that new strategies be developed to effectively incor-
         porate the positive elements of the existing approaches reviewed in this paper and to
         develop new approaches that can significantly reduce costs while achieving positive
         energy gains and net negative CO 2 production. Regardless of what set of technological
         approaches is used for harvest, extraction, and conversion, these solutions must be
         environmentally sustainable while being sufficiently inexpensive to make microalgal
         biofuels competitive with petroleum-based transportation fuels.



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