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134    Advances in textile biotechnology


                                                              Glycoprotein

                                                                      2+
                                                                    Ca  bridges
                                                                     between
                                                                   acidic pectins


                                     Ca 2+  Ca 2+  Ca 2+           Hemicellulose
                     Cellulose
                     microfibril


                                                                    Acidic pectin


                                                             Esterified pectin
                     6.2  Connections between cellulose and non-cellulosic constituents of
                     the cotton primary wall (Agrawal et al., 2007).





                      Cuticle  Primary wall  Secondary wall  Lumen


                      Waxes

                              Pectin, protein,
                              hemicelluloses
                              and metal ions


                                              Cellulose


                     6.3  Model of cotton structure (Agrawal, 2005, after Kim, 2001).




                The outer layer of the cotton fiber, consisting of the chemical mixture
              mentioned previously, is the protective coating that all plant cells have
              evolved to protect themselves from environmental degradation. Cotton
              lacks the lignin that plant cells generally possess. The wax that is contained
              in the thin outer coating cuticle of the cotton fi ber is a complex mixture of
              fatty acids, and high-molecular-weight alcohols and esters. This cuticle has
              been estimated to be approximately 12 nm thick (Ryser and Holloway,
              1985). A number of studies have analyzed the mixture of compounds in the
              cuticle. Some references classify these as saponifiable and non-saponifi able,




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