Page 234 - Algae

Algal Culturing 217

TABLE 6.2
BG11 Medium Composition

Reagents Per Liter
a
NaNO 3 1.5 g
K 2 HPO 4 * 3H 2 O 0.004 g
MgSO 4 * 7H 2 O 0.075 g
CaCl 2 * 2H 2 O 0.027 g
Citric acid (C 6 H 8 O 7 ) 0.006 g
Ammonium ferric citrate (C 6 H 8 O 7 * nFe * nNH 3 ) 0.006 g
EDTANa 2 Mg 0.001 g
Na 2 CO 3 0.02 g
Microelement stock solution 1 ml
Microelement Stock Solution
2.860 g
H 3 BO 3
MnCl 2 * 4H 2 O 1.810 g
ZnSO 4 * 7H 2 O 0.222 g
Na 2 MoO 4 * 2H 2 O 0.390 g
CuSO 4 * 5H 2 O 0.079 g
Co(NO 3 ) 2 * 6H 2 O 0.0494 g
pH ¼ 7.4
a
To be omitted for N 2 -fixing cyanobacteria

TABLE 6.3
Diatom Medium Composition

Reagents Per Liter
Ca(NO 3 ) 2* 4H 2 O 20 mg
KH 2 PO 4 12.4 mg
MgSO 4* 7H 2 O 25 mg
NaHCO 3 15.9 mg
EDTA FeNa 2.25 mg
EDTA Na 2 2.25 mg
2.48 mg
H 3 BO 3
MnCl 2* 4H 2 O 1.39 mg
(NH 4 ) 6 Mo 7 O 24* 4H 2 O 1.0 mg
Biotin (Vitamin H) 0.04 mg
Thiamine HCl (Vitamin B 1 ) 0.04 mg
Cyanocobalamin (Vitamin B 12 ) 0.04 mg
Na 2 SiO 3* 9H 2 O 57 mg
pH ¼ 6.9