Page 237 - Algae

220 Algae: Anatomy, Biochemistry, and Biotechnology

TABLE 6.6
Cramer and Myers Medium Composition

Reagents Per Liter
1g
(NH 4 ) 2 HPO 4
1g
KH 2 PO 4
MgSO 4* 7H 2 O 0.2 g
CaCl 2 0.02 g
Sodium Citrate (Na 3 C 6 H 5 O 7 * 2H 2 O) 0.8 g
Fe 2 (SO 4 ) 3 * nH 2 O 3 mg
MnCl 2 * 4H 2 O 1.8 mg
CoSO 4 * 7H 2 O 1.5 mg
ZnSO 4 * 7H 2 O 0.4 mg
Na 2 MoO 4 * 2H 2 O 0.2 mg
CuSO 4 * 5H 2 O 0.02 mg
2.48 mg
H 3 BO 3
Thiamine HCl (Vitamin B 1 ) 0.5 mg
Cyanocobalamin (Vitamin B 12 ) 0.02 mg
Carbon Sources (Choose One)
Glucose † 10 g
Succinic acid (C 4 H 6 O 4 ) † 5g
Glutamic acid (C 5 H 9 NO 4 ) † 5g
Sodium Acetate (C 2 H 3 O 2 Na * 5H 2 O) †† 1.64 g
† pH ¼ 3.4
†† pH ¼ 6.8