Page 221 - Analytical method for food addtives

E310–12: Gallates 147

10
6 7 8 9
V
UV at 280 nm Electrochemical oxidation potential +0.8 versus Ag–AgCl UV at 284 nm UV at 284 nm 3 electrodes, reference electrode at V, sensitivity 0.90 nA is 50

Gradient elution system of 5 % acetic acid and MeOH–acetonitrile (50:50). Flow rate 1 mL/min Water–MeOH (10:90), M LiClO 4 , pH 5.5 0.01 at 50 µL/min M SDS, 2.5 % 0.1 mM n-propanol and 10 phosphate at pH 3. Flow rate 1 mL/min M SDS, 6.6 % 0.090 mM n-propanol and 10 phosphate at pH 3. Flow rate 1 mL/min Isocratic CH 3

CrestPac C18S mm) (4.6 × 150 Kromasil C18 mm, mm × 1 (300 5 µm) Spherisorb ODS-2 mm, 5 µm) (125 × 4 and guard column mm, 10 µm) (35 × 4.6 Spherisorb ODS-2 mm, 5 µm) (125 × 4 and guard column (35 × 4.6 mm, 10 µm) Lichrocart RP18 (250 × 4 mm, 5 µm)

Extracted with acetonitrile–2- propanol–ethanol (2:1:1). Extract in freezer for 1 h then filtered. Filtrate concentrated under vacuum. 10 µL injected MeOH added to sample and sonicated and re-extracted. Extracts filtered on a SepPak silica cartridge connected to syringe. 0.2 µL injected Microemulsion of oil prepared by mixing 5 %

Foods Foods and drugs Edible oils Dairy products and dietetic supplements Foods

HPLC LC LC LC HPLC-ECD