Page 41 - Analytical method for food addtives
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18  Analytical methods for food additives


              Quantitative determination: HPLC
              Load 20 µL of sample extract onto column and use gradient (linear) elution to
              achieve optimum separation. The same HPLC conditions were used as for sunset
              yellow in soft drinks but the detector was set at 520 nm for azorubine.



              Analysis of beverages 3
              The same sample preparation, analysis and HPLC conditions as used for sunset
              yellow (Chapter 1, Appendix) were used to determine azorubine.
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