Page 42 - Analytical method for food addtives

7
nm
520
mM octylamine/
Water–acetonitrile (7:3)
containing 5
ODS-2 with
Spherisorb
Column Reference Detection Mobile phase 2 Diode-array mL/min) Gradient elution (1.5 Spherisorb C8 nm at 520 with phosphate buffer containing cetylpyridinium chloride, acetonitrile and methanol 3 nm 520 mL/min) Gradient elution (2 Nova-Pak C18 M sodium using methanol and 0.1 phosphate buffer at pH 7 4 nm 520 mL/min) Grad
Summary of methods for azorubine in foods Sample Matrix preparation/extraction Ion pairs with cetylpyridinium Lemonade, chloride from aqueous solutions cake crumb, into n-butanol skimmed milk Diluted with water and filtered Bitters Diluted with water and filtered Beverages, gelatine, syrups Shaken with 5 Yogurt added and shak

Table 2.1 (a) Method IP-RP-HPLC RP-HPLC HPLC HPLC HPLC