Page 81 - Analytical method for food addtives
P. 81

E200–3: Sorbic acid and its salts  45




                            13           14


                            nm           nm
                            UV at 255    UV at 258




                            % ammonium acetate  %  NH 2 SO 4 –CH 3 CN




                         temperature  90  buffer with 10  acetonitrile  0.01  (75:25)






                              mm,
                            Supelcosil LC-18  mm × 4.6  (250  µm)  5  Divinyl benzene-  styrene copolymer  (DVB-H)







                            Liquid samples dilute 10 fold in acetonitrile/ammonium acetate buffer solution. Solid samples blended with same buffer solution 1:5 followed by dilution as for liquid samples  Yogurt samples treated with potassium ferricyanide (III) and zinc sulphate. Non- alcoholic beverages and fruit juices diluted and filtered













                                         Yogurt, non-  beverages and  fruit juices

                            Foods          alcoholic







                            HPLC         HPLC
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