Page 194 - Cascade biocatalysis

170 7 Synergies of Chemistry and Biochemistry for the Production of -Amino Acids

Table 7.1 Kinetic parameters of Atβcar for several precursors of α, β, γ,and δ amino acids.

−1
Substrate K (mM) k (s ) k /K p (%)
m cat cat m i
O
H N N COOH 4.75 ± 0.43 21.88 ± 0.76 4.61 ± 0.58 3
2
H
N-carbamoyl-glycine
O CH 3
0.26 ± 0.04 6.27 ± 0.28 24.12 ± 4.59 17
H N N COOH
2
H
α N-carbamoyl-L-alanine
Ph
O
0.89 ± 0.05 5.81 ± 0.11 6.53 ± 0.48 5
H N N COOH
2
H
N-carbamoyl-L-phenylalanine
CH SCH
CH 2 2 3
O
H N N COOH 0.11 ± 0.01 8.64 ± 0.13 78.55 ± 7.81 55
2
H
N-carbamoyl-L-methionine
O
COOH 2.14 ± 0.19 25.71 ± 1.01 12.01 ± 1.22 8
H N N
2
H
N-carbamoyl-β-alanine
O CH 3
COOH
H N N 3.44±0.76 0.21±0.01 0.06±0.02 0
2
H
N-carbamoyl-β-homoalanine
O
COOH
H N N
2
H 0.07 ± 0.02 0.15 ± 0.01 2.14 ± 0.82 1
NH 2
β N-cabamoyl-α-amino-β-alanine
O
COOH
H N N 6.59 ± 0.55 24.39 ± 1.07 3.70 ± 0.47 3
2
H
CH 3
N-carbamoyl-α-methyl-β-alanine
O
COOH
H N N 84.38 ± 23.07 0.13 ± 0.00 1.54e−3 ± 0.00 0
2
H
Ph
N-carbamoyl-α-phenyl-β-alanine