Page 208 - Color Atlas of Biochemistry

Basics 199

A. Isolation of cell organelles

Homo- Filter
genize

Slice Gauze

Whole
Tissue
cells,
connective
tissue
Potter
Buffer homo-
genizer
Centrifuge

g = 300 000 g = 100 000 g = 15 000 g = 600
120' 60' 15' 10'

Cytosol

Super-
natant
Centrifuge
tube
Pellet

Ribosomes Plasma membrane Mitochondria Nucleus
Viruses ER fragments Lysosomes Cytoskeleton
Macromolecules Small vesicles Peroxisomes
Microsomal (Plants:
fraction chloroplasts)

B. Marker molecules

Golgi complex Nucleus Ribosomes Plasma membrane
+ +
α-Mannosidase II DNA rRNA Na /K ATPase
3.2.1.24 3.6.1.37
Phosphodiesterase I
3.1.4.1
Endoplasmic
reticulum
Lysosome
Glucose 6-phospha- β-N-Acetylhexos-
tase 3.1.3.9 aminidase 3.2.1.52
RNA β-Galactosidase
3.2.1.23
Mitochondrion
Succinate dehydro- Endosome
genase 1.3.5.1 Uptake of
Cytochrome-c peroxidase
oxidase 1.11.1.7
1.9.3.1
Cytosol
Peroxisome L-Lactate
Catalase dehydrogenase
1.11.1.6 1.1.1.27

Cell fraction Marker enzyme

Koolman, Color Atlas of Biochemistry, 2nd edition © 2005 Thieme
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