Page 343 - Fundamentals of Light Microscopy and Electronic Imaging

326 APPENDIX II

Chapter 10: DIC Microscopy

Materials

24 coverslips of cultured cells
serum-free MEM culture medium supplemented with 10 mM HEPES, pH 7.2
filter paper
petroleum jelly
paper wipes (Kimwipes)
lens cleaner, water, immersion oil
Pasteur pipettes and rubber bulbs
plastic beakers for discarded medium
fine forceps

Chapter 11: Fluorescence Microscopy

Materials

24 coverslips of cultured cells
serum-free MEM culture medium supplemented with 10 mM HEPES, pH 7.2
filter paper
petroleum jelly
paper wipes (Kimwipes)
lens cleaner, water, immersion oil
Pasteur pipettes and rubber bulbs
plastic beakers for discarded dyes and medium
fine forceps
chlorophyll extract
fluorescein solution, 10 mg fluorescein in 1 L water
delipidated BSA (Sigma A-6003)
long-wave UV lamp (blacklight)
light box
spectrometers
cardboard sheet with 1 cm wide slit
optical bench
IR cut filter
holographic diffraction grating
1 mm slit aperture
xenon arc lamp and power supply