Page 6 - Fundamentals of Light Microscopy and Electronic Imaging

CONTENTS

Preface xi

1. FUNDAMENTALS OF LIGHT MICROSCOPY 1
Overview 1
Optical Components of the Light Microscope 1
Note: Inverted Microscope Designs 3
Aperture and Image Planes in a Focused, Adjusted Microscope 4
Note: Using an Eyepiece Telescope to View the Objective Back Aperture 5
Koehler Illumination 6
Adjusting the Microscope for Koehler Illumination 7
Note: Summary of Steps for Koehler Illumination 7
Note: Focusing Oil Immersion Objectives 11
Precautions for Handling Optical Equipment 11
Exercise: Calibration of Magnification 12

2. LIGHT AND COLOR 15
Overview 15
Light as a Probe of Matter 15
Light as Particles and Waves 18
The Quality of Light 20
Properties of Light Perceived by the Eye 21
Physical Basis for Visual Perception and Color 22
Positive and Negative Colors 24
Exercise: Complementary Colors 26

3. ILLUMINATORS, FILTERS, AND ISOLATION
OF SPECIFIC WAVELENGTHS 29
Overview 29
Illuminators and Their Spectra 29
v