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Page 63

2—
Reagent for UV-VIS Detection

Kiyoshi Zaitsu∗, Masaaki Kai and Kenji Hamase
Faculty of Parmaceutical Science, Kyushu University, Japan

2.1 Introduction 64

2.2 Label of Amines and Amino Acids (-NH , NH) 65
2

2.2.1 Nitrobenzenes 65

2.2.1.1 1-Fluoro-2,4-dinitrobenzene (FDNB, 2,4-Dinitro-1- 65
fluorobenzene, Sanger reagent)

2.2.1.2 4-Fluoro-3-nitrotrifluoromethylbenzene (4-Fluoro-3- 66
nitrobenzotrifluoride) (FNBT)

2.2.1.3 2,4,6-Trinitrobenzene-1-sulfonic Acid (TNBS) 67

2.2.1.4 Na-(2,4-Dinitro-5-fluorophenyl)-L-alanine amide 67
(FDAA,1-Fluoro-2,4-diintrophenyl-5-L-alanine Amide,
Marfey's Reagent)

2.2.2 Acetic Anhydride 67

2.2.3 Isocyanates and Isothiocyanates 67

2.2.3.1 Isocyanates 67

2.2.3.2 Phenyl Isothiocyanate (PITC, Edman Reagent) 68

2.2.3.3 Butylisothiocyanate (BITC) 70

2.2.3.4 4-N,N-Dimethylaminoazobenzene-4'-isothiocyanate 70
(DABITC)

2.2.4 1,2-Naphthoquinone-4-sulfonate (NQS) 70

2.2.5 Acyl Chlorides 71

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