Page 149 - Modern Derivatization Methods for Separation Sciences
P. 149
Document Página 2 de 2
2.5 Label of Reducing Carbohydrate 89
2.5.1 Reductive Amination 89
2.5.2 l-Phenyl-3-methyl-5-pyrazolone 91
2.5.3 Post-column Derivatizing Reagent 92
2.6 Label of Thiol (-SH) 92
2.6.1 2-Halopyridinium Salt 92
2.6.2 Disulfhide Reagent 93
2.6.3 Other Reagents 94
2.7 Labelling of Other Compounds 94
2.7.1 1-(2-Pyridyl)-piperazine (PYP) 94
2.7.2 Diethyldithiocarbamate (DDTC) 94
2.7.3 9-Methylamino-methylanthracene (MAMA) 95
References 95
2.1—
Introduction
Whenever a new or a more sensitive and selective method has been developed for the detection and
determination of biologically important compounds, drugs or their metabolites, biopolymers, and also
environmental pollutants, important scientific knowledge has evolved to a greater or lesser extent. In
the 1970s and 1980s, various derivatization methods for ultraviolet and visible light (UV-VIS)
absorption detection have been developed [1-5] for high-performance liquid chromatography (HPLC),
and many of the fundamental UV-VIS derivatizing reagents
http://emedia.netlibrary.com/nlreader/nlreader.dll?bookid=17968&filename=Page_64.html 30/09/2003
