Page 153 - Modern Derivatization Methods for Separation Sciences

Document Página 2 de 2

The dry residue obtained from a sample solution containing putrescine, spermidine and spermine
together with diaminooctane (IS) was redissolved in 0.1 ml of 1 M Na CO . The solution was mixed
3
2
with 0.3 ml of FNBT reagent (10 µ1 FNBT/ml DMSO) and kept at 60 °C for 20 min. To the resulting
mixture, 40 µ1 of 1 M histidine in 1 M

Fig. 2.2.
The reaction of FNBT with primary amines.

http://emedia.netlibrary.com/nlreader/nlreader.dll?bookid=17968&filename=Page_66.html 30/09/2003