Page 157 - Modern Derivatization Methods for Separation Sciences

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S-Alk(en)yl-L-cysteine sulfoxides (Fig. 2.8) in various Allium can be determined for the allien content
of various samples of garlic acid by use of the Waters Pico-Tag method, which employs PITC as
derivatization reagent. The HPLC system used for the Pico-Tag method was a Waters chromatograph
consisting of a UV detector with a wavelength fixed at 254 nm [33]. The origin of this method is
developed by Bidlingmeyer et al. in Waters Associates. In 1984 they had

Fig. 2.7.
The formation of phenylthiocarbamoyl derivatives by reaction of
phenylisothiocyanate with amino acids.

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