Page 239 - Multidimensional Chromatography

Foods, Flavours and Fragrances Applications 233

Figure 10.8 Application of the heart-cutting method for the determination of sugars in
molasses: (a) exclusion chromatogram of molasses: (b) normal-phase chromatogram of
molasses heart-cut. The conditions were as follows: exclusion chromatography, MicroPak
TSK 2000 PW column (30 cm 7.5 mm i.d.); 0.1% acetic acid in water at 1.2 ml/min; detec-
tion, UV, 192 nm, 0.5 a.u.f.s.; injection volume, 100 l: normal-phase chromatography,
MicroPak NH2-10 column (30 cm 4 mm i.d.); acetonitrile–water (65 : 35) at 2.8 ml/min;
detection, UV, 192 nm, 0.2 a.u.f.s.; trapping loop volume, 50 l. Reprinted from Journal of
Chromatography, 206, J. A. Apffel et al., ‘Automated on-line multi-dimensional high perfor-
mance liquid chromatographic techniques for the clean-up and analysis of water-soluble sam-
ples’, pp. 43–57, copyright 1981, with permission from Elsevier Science.