Page 347 - Multidimensional Chromatography

338 Multidimensional Chromatography

Kinghorn et al. used MDGC and electron-capture detection (ECD) to determine
seven specific chlorobiphenyl congeners (key congeners) which co-eluted with other
components in the mixture of an Arochlor standard (22). Figure 13.1 shows the sepa-
ration from the first column (BPX5) with flame-ionization detection (FID) and the
separation of the different cuts in the second column (HT8) with ECD. The separa-
tion of most congeners is good.
The chirality of the PCB congeners has also been taken into account in this
separation. From a total of 209 congeners, 78 are axially chiral in their nonplanar

Figure 13.1 Monitor (FID) (a) and analytical (ECD) (b) channel responses for PCB con-
geners in Aroclor 1254, showing selection of the six heart-cut events: First columns, HT8;
second columns, BPX5. Reprinted from Journal of High Resolution Chromatography, 19,
R. M. Kinghorn et al., ‘Multidimensional capillary gas chromatography of polychlorinated
biphenyl marker compounds’, pp. 622–626, 1996, with permission from Wiley-VCH.