Page 349 - Multidimensional Chromatography

340 Multidimensional Chromatography

Figure 13.2 MDGC-ECD chromatograms of PCB fractions from sediment samples, demon-
strating the separation of the enantiomers of (a) PCB 95, (b) PCB 132, and (c) PCB 149; non-
labelled peaks were not identified. Reprinted from Journal of Chromatography, A 723,
A. Glausch et al., ‘Enantioselective analysis of chiral polychlorinated biphenyls in sediment
samples by multidimensional gas chromatography–electron-capture detection after steam dis-
tillation–solvent extraction and sulfur removal’, pp. 399–404, copyright 1996, with permis-
sion from Elsevier Science.

Figure 13.4 shows the standard chromatogram obtained by using this method. In this
sample, isoprene and major interference compounds, i.e. 2-methyl pentane and
1
1
hexane, were spiked at 1 g l , plus DMS at 10 g l .
When using one-dimensional GC (1D-GC), the analysis took about 60 min
(shown in the inset to Figure 13.4), while with the use of two-dimensional (2D-GC)
it took only about 12 min.