Page 425 - Tandem Techniques
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chromatograms obtained from a blood sample, monitored by both a UV detector and the flame AAS
detector employing the serpentine interface, is shown in Figure 10.2.
Figure 10.2
Chromatograms of a Blood Sample Monitored
by the UV Detector and the Atomic Absorption
Spectrometer with the Serpentine Tube Interface
It is seen from the chromatogram monitored by UV absorption that the mixture is complex, and the
resolution obtained from the column is rather poor. The number and nature of the peaks shown are
almost impossible to identify. In contrast, the flame AAS unambiguously picks out the peak containing
iron, and the width of the iron peak clearly substantiates the efficacy of the serpentine interface.
