Page 476 - Tandem Techniques
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A photograph of a commercial TLC scanner manufactured by CAMAG is shown in Figure 12.2.
Finally, in the measuring process, extreme care must be taken to ensure the spot is located in the exact
center of the measuring beam or, again, errors will result
Most scanning densitometers are suitable for use with both TLC plates and also electrophoretic stains,
and can deal with objects (plates or gel sheets) 200 x 200 mm in size. The spot can be monitored by
either reflected light, emitted light, or transmitted light. The wavelengths available range from 190 nm
to 800 nm and scanning speeds as fast as 100 mm/s are available. The spatial resolution can be selected
from 25 to 200 mm. A diagram of the optical layout is shown in Figure 12.3.
Figure 12.3
The Optical layout of the CAMAG TLC Scanner
Courtesy of CAMAG Inc.
