Page 61 - Tandem Techniques
P. 61
Page 43
flow. In 'plug' flow there is virtually no band dispersion resulting from resistance to mass transfer
effects. The capillary electrophoretic apparatus consists of two reservoirs, each containing electrolyte,
and fitted with electrodes. Each reservoir is inter-connected by fused quartz capillary tubing dipping
into each reservoir. A third reservoir, containing another electrode, is connected by a 'T' to one end of
the capillary, through which the sample is applied to the tube. At the other end of the tube is the
detector. The most common detector is a UV absorption system measuring the intensity of the light
passing through a cleaned section of the tube using fiber optics. Using the same physical system
detection by fluorescence measurement has also been found satisfactory.
References
1. A. T. James and A. J. P. Martin, Biochemist. J., 50(1952)679.
2. R. P. W. Scott in Gas Chromatography 1958, D. H. Desty (Ed.), Butterworth London (1958)189.
3. M. J. E. Golay, idem, 36.
4. D. H. Desty, A. Goldup and B. F. Wyman, J. Inst. Petrol., 45(1959)287.
5. R. D. Dandenau and E. M. Zenner. J. High Res. Chromatogr., 2(1979)351.
6. K. L. Ogan, C. Reese and R. P. W. Scott, J. Chromatogr. Sci., 20(1982)425.
7. K. L. Ogan, U. S. A. Patent Application (pending).
8. J. Stuff, M. Wojtas and P. Uden, Analyst (in press).
9. D. R. Biswas, ITT, Virginia. Private Communication.
10. K. Grob Jr., J. Chromatogr., 237(1982)15.
11. J. V. Hinshaw, Jr. and F. J. Yang, J. High Resol. Chromatogr., 6(1983)554.
12. E. Katz and R. P. W. Scott, J. Chromatogr., 253(1982)159.
13. R. P. W. Scott and P. Kucera, J. Chromatogr. 169(1979)51.
14. R. P. W. Scott and P. Kucera, J. Chromatogr. 125(1976)251.
15. A. Alhedai, D. E. Martire and R. P. W. Scott, Analyst, 114(1989)869.
16. H. Zhou, C. F. Poole, J. Triska and A. Zlatkis, J. High Resolution Chromatog., 3(1980)440.
16. S. O. Akapo, R. P. W. Scott and C. F. Simpson, J. Chromatogr. Sci., 14(1991)217.
17. C. H. Lochmuller and D. R. Wilder, J. Chromatogr. Sci., 17(1979)574.
18. R. K. Gilpin and J. A. Squires, J. Chromatogr. Sci. 20(1982)345.
19. R. P. W. Scott and C. F. Simpson, J. Chromatogr., 197(1980)11.