Page 160 - The Biochemistry of Inorganic Polyphosphates

WU095/Kulaev
WU095-08
Peculiarities of polyphosphate metabolism
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(a) 2000 1
PolyP (µg (100 ml) −1 ) 1500 2

1000

300
100
(b) 500
0.02
2
0.01
1
(c)
0.02
1
0.01 2

(d) 2.0 1
Activity (E (100 ml −1 )) 1.5 2

1.0

(e) 0.5
0.15
2
0.1
0.05 1
(f)
0.15
2
0.1
0.05 1
0.01
0 1 2 3 4 5
Time (d)

Figure 8.12 Changes in polyphosphate and polyphosphate-metabolising enzymes during the de-
velopment of a culture of Propionibacterium shermanii under normal conditions, and in the pres-
ence of polymyxin M (Kulaev et al., 1973a): (1) control conditions; (2) polymyxin M: (a) total
PolyP; (b) 1,3-diphosphoglycerate–polyphosphate phosphotransferase; (c) polyphosphate kinase; (d)
polyphosphate–glucokinase; (e) tripolyphosphatase; (f ) exopolyphosphatase with PolyP 290 .