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W idefield Raman Imaging of Cells and T issues 187

6.7 Conclusions
Widefield Raman imaging, along with other Raman spectroscopic-
based modalities, are effective tools in the study of biological sam-
ples. The challenge is to derive useful information from all of the
spectral data created by these methods. Chemometrics provides
powerful analytic techniques for the evaluation of Raman spectra,
as evidenced by the many different types of cells and tissue that
can be discriminated from each other based on classification mod-
els that were created using one of many multivariate statistical
analytical techniques. Each technique has its advantages and limi-
tations; the best choice for analysis depends on each situation and
what is known about the sample data beforehand. Ultimately,
Raman spectroscopic methods have a promising future in the
objective diagnosis of disease and decision making regarding bio-
logical samples.

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