Page 7 - Vibrational Spectroscopic Imaging for Biomedical Applications
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Contents
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xiii
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xv
1 Toward Automated Breast Histopathology Using
Mid-IR Spectroscopic Imaging F. Nell Pounder and
R. Bhargava . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.1.1 FT-IR Imaging . . . . . . . . . . . . . . . . . . . 4
1.1.2 FT-IR Spectroscopic Characterization
of Cells and Tissues . . . . . . . . . . . . . . . 5
1.1.3 FT-IR Imaging for Pathology . . . . . . . 6
1.1.4 High-Throughput Sampling . . . . . . . 8
1.1.5 Modified Bayesian Classifi cation and
Automated Tissue Histopathology . . . 8
1.2 Materials and Methods . . . . . . . . . . . . . . . . . . . 10
1.2.1 Models for Spectral Recognition and
Analysis of Class Data . . . . . . . . . . . . 11
1.2.2 Automated Metric Selection and
Classification Protocol Optimization . . . 12
1.2.3 Spectral Metrics and Biochemical
Basis . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
1.2.4 Validation and Dependence on
Experimental Parameters . . . . . . . . . . 15
1.2.5 Application for Cancer
Pixel Segmentation . . . . . . . . . . . . . . . 18
1.2.6 Application for Patient Cancer
Segmentation . . . . . . . . . . . . . . . . . . . . 21
1.3 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
2 Synchrotron-Based FTIR Spectromicroscopy and
Imaging of Single Algal Cells and Cartilage
Michael J. Nasse, Eric Mattson, Claudia Gohr, Ann
Rosenthal, Simona Ratti, Mario Giordano, and
Carol J. Hirschmugl . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
2.2 IR Environmental Imaging . . . . . . . . . . . . . . . . 31
2.2.1 Beamline Design and
Implementation . . . . . . . . . . . . . . . . . 32
2.2.2 Initial Measurements with IRENI . . . 35
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