Page 235 - Algae Anatomy, Biochemistry, and Biotechnology

218 Algae: Anatomy, Biochemistry, and Biotechnology

TABLE 6.4
DY-III Medium Composition

Reagents Per Liter
MgSO 4* 7H 2 O 50 mg
KCl 3 mg
NH 4 Cl 2.68 mg
NaNO 3 20 mg
H 3 BO 3 0.8 mg
Na 2 EDTA * 2H 2 O 8 mg
Na 2 SiO 3* 9H 2 O 14 mg
FeCl 3* 6H 2 O 1 mg
75 mg
CaCl 2
MES buffer 200 mg
Microelement stock solution 1 ml
Vitamin solution 1 ml
Microelement Stock Solution
MnCl 2* 4H 2 O 200 mg
ZnSO 4* 7H 2 O 40 mg
CoCl 2* 6H 2 O 8 mg
Na 2 MoO 4* 6H 2 O 20 mg
Na 3 VO 4* nH 2 O 2 mg
2mg
H 2 SeO 3
Vitamin Solution
Biotin (Vitamin H) 0.5 mg
Thiamine HCl (Vitamin B 1 ) 100 mg
Cyanocobalamin (Vitamin B 12 ) 0.5 mg
pH ¼ 6.7