Page 240 - Algae Anatomy, Biochemistry, and Biotechnology
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Algal Culturing 223
TABLE 6.9
Mes-Volvox Medium Composition
Reagents Per Liter
MES buffer 1.95 g
Ca(NO 3 ) 2* 4H 2 O 117.8 mg
Na 2 -glycerophosphate * 5H 2 O 60 mg
MgSO 4* 7H 2 O 40 mg
KCl 50 mg
NH 4 Cl 26.7 mg
Biotin (Vitamin H) 0.0025 mg
Cyanocobalamin (Vitamin B 12 ) 0.0015 mg
Microelement stock solution 6 ml
Microelement Stock Solution
Na 2 EDTA 750 mg
FeCl 3 * 6H 2 O 97 mg
MnCl 2 * 4H 2 O 41 mg
5mg
ZnCl 2
CoCl 2 * 6H 2 O 2 mg
Na 2 MoO 4 * 2H 2 O 4 mg
pH ¼ 6.7
