Page 239 - Algae Anatomy, Biochemistry, and Biotechnology
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222 Algae: Anatomy, Biochemistry, and Biotechnology
TABLE 6.8
Bold Basal Medium Composition
Reagents Per Liter
175 mg
KH 2 PO 4
CaCl 2 * 2H 2 O 25 mg
MgSO 4 * 7H 2 O 75 mg
NaNO 3 250 mg
K 2 HPO 4 75 mg
NaCl 25 mg
H 3 BO 3 11.42 mg
Microelement stock solution 1 ml
Solution 1 1 ml
Solution 2 1 ml
Microelement Stock Solution
ZnSO 4 * 7H 2 O 8.82 g
MnCl 2 * 4H 2 O 1.44 g
0.71 g
MoO 3
CuSO 4 * 5H 2 O 1.57 g
Co(NO 3 ) 2 * 6H 2 O 0.49 g
Solution 1
Na 2 EDTA 50 g
KOH 3.1 g
Solution 2
4.98 g
FeSO 4
H 2 SO 4 (Conc.) 1 ml
pH ¼ 6.8