Page 239 - Algae Anatomy, Biochemistry, and Biotechnology
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222                                   Algae: Anatomy, Biochemistry, and Biotechnology



                                    TABLE 6.8
                                    Bold Basal Medium Composition

                                    Reagents                             Per Liter
                                                                          175 mg
                                    KH 2 PO 4
                                    CaCl 2 * 2H 2 O                        25 mg
                                    MgSO 4 * 7H 2 O                        75 mg
                                    NaNO 3                                250 mg
                                    K 2 HPO 4                              75 mg
                                    NaCl                                   25 mg
                                    H 3 BO 3                             11.42 mg
                                    Microelement stock solution             1 ml
                                    Solution 1                              1 ml
                                    Solution 2                              1 ml

                                    Microelement Stock Solution
                                    ZnSO 4 * 7H 2 O                       8.82 g
                                    MnCl 2 * 4H 2 O                       1.44 g
                                                                          0.71 g
                                    MoO 3
                                    CuSO 4 * 5H 2 O                       1.57 g
                                    Co(NO 3 ) 2 * 6H 2 O                  0.49 g
                                    Solution 1
                                    Na 2 EDTA                              50 g
                                    KOH                                   3.1 g
                                    Solution 2
                                                                          4.98 g
                                    FeSO 4
                                    H 2 SO 4 (Conc.)                        1 ml
                                    pH ¼ 6.8
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