Page 246 - Algae Anatomy, Biochemistry, and Biotechnology

Algal Culturing 229

TABLE 6.11
Walne’s Medium Composition

Reagents Per Liter Seawater
Solution A 1.0 ml
Solution C 0.1 ml
Solution D (to add for diatoms) 2.0 ml
Solution A
FeCl 3* 6H 2 O 1.3 g
MnCl 2* 4H 2 O 0.4 g
H 3 BO 3 33.6 g
Na 2 EDTA 45.0 g
NaH 2 PO 4* 2H 2 O 20.0 g
100.0 g
NaNO 3
Solution B 1 ml
Solution B per 100 ml
2.1 g
ZnCl 2
CoCl 2* 6H 2 O 2.0 g
(NH 4 ) 6 Mo 7 O 24 * 4H 2 O 0.9 g
CuSO 4* 5H 2 O 2.0 g
Concentrated HCl 10 ml
Solution C per 200 ml
Thiamine HCl (Vitamin B 1 ) 0.2 g
Cyanocobalamin (Vitamin B 12 ) 10 mg
Solution D (per liter)
Na 2 SiO 3* 5H 2 O 40.0 g