Page 250 - Algae Anatomy, Biochemistry, and Biotechnology

Algal Culturing 233

TABLE 6.17
ESAW Medium Composition

Reagents Per Liter
NaCl 21.19 g
3.55 g
Na 2 SO 4
KCl 0.599 g
Na 2 HCO 3 0.174 g
KBr 0.0863 g
H 3 BO 3 0.023 g
NaF 0.0028 g
MgCl 2* 6H 2 O 9.592 g
CaCl 2* 2H 2 O 1.344 g
0.0218 mg
SrCl 2
46.7 mg
NaNO 3
NaH 2 PO 4* H 2 O 3.09 mg
Na 2 SiO 3* 9H 2 O 30 mg
Metal Stock I 1 ml
Metal Stock II 1 ml
Vitamin Solution 1 ml
Metal Stock I
Na 2 EDTA * 2H 2 O 3.09 g
FeCl 3* 6H 2 O 1.77 g
Metal Stock II
Na 2 EDTA * 2H 2 O 2.44 g
ZnSO 4* 7H 2 O 0.073 g
CoSO 4* 7H 2 O 0.016 g
MnSO 4* 4H 2 O 0.54 g
Na 2 MoO 4* 2H 2 O 1.48 mg
Na 2 SeO 3 0.173 mg
NiCl 2* 6H 2 O 1.49 mg
Vitamin Solution
Biotin (Vitamin H) 1 mg
Thiamine HCl (Vitamin B 1 ) 100 mg
Cyanocobalamin (Vitamin B 12 ) 2 mg
pH ¼ 8.2