Page 248 - Algae Anatomy, Biochemistry, and Biotechnology

Algal Culturing 231

TABLE 6.14
PCR-S11 Medium Composition

Reagents Per Liter Seawater
2.68 mg
(NH 4 ) 2 SO 4
8mg
Na 2 EDTA/FeCl 3
Na 2 PO 4 (pH 7.5) 14 mg
HEPES-NaOH (pH 7.5) 200 mg
Microelement stock solution 0.5 ml
Microelement Stock Solution
H 3 BO 3 18.549 mg
MnSO 4* H 2 O 10.140 mg
ZnSO 4* 7H 2 O 1.725 mg
(NH 4 ) 6 Mo 7 O 24* 4H 2 O 0.494 mg
CuSO 4* 5H 2 O 0.749 mg
Co(NO 3 ) 2* 6H 2 O 0.873 mg
VSO 5* 5H 2 O 0.098 mg
Na 2 WO 4* 2H 2 O 0.198 mg
KBr 0.714 mg
KI 0.498 mg
Cd(NO 3 ) 2* 6H 2 O 0.925 mg
NiCl 2* 6H 2 O 0.713 mg
Cr(NO 3 ) 3* 9H 2 O 0.240 mg
0.333 mg
SeO 2
KAl(SO 4 ) 2* 12H 2 O 2.846 mg
Cyanocobalamin (Vitamin B 12 ) 10 mg
pH ¼ 7.5

TABLE 6.15
f/2 Medium Composition

Reagents Per Liter Seawater

NaNO 3 0.075 g
NaH 2 PO 4* H 2 O 0.005 g
Microelement stock solution 1 ml
Vitamin solution 1 ml
Microelement Stock Solution
FeCl 3* 6H 2 O 3.150 g
Na 2 EDTA 4.160 g
MnCl 2* 4H 2 O 0.180 g
CoCl 2* 6H 2 O 0.010 g
CuSO 4* 5H 2 O 0.010 g
ZnSO 4* H 2 O 0.022 g
Na 2 MoO 4* 2H 2 O 0.006 g
Vitamin Solution
Biotin (Vitamin H) 0.5 mg
Thiamine HCl (Vitamin B 1 ) 100 mg
Cyanocobalamin (Vitamin B 12 ) 0.5 mg
pH ¼ adjust to 8.0 with 1 M NAOH or HCl