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uncertainties regarding future production technologies, future costs of liquid autotrophic
        microalgal biofuels are highly uncertain.     22,23  It has been argued that a successful strategy to
        lower the prices of future microalgal biofuel is to use microalgal biomass as an input for
        biorefineries, which produce, apart from liquid biofuel, a variety of high-value substances as
        coproducts.23-26 A problem with this strategy is that to make a substantial contribution to
        future energy supply, the production volume of biofuels should be large. This may easily lead

        to an oversupply of current high-value substances. This has already happened to glycerol,
        which is produced as a coproduct of biodiesel. The price thereof has dropped dramatically.              27

        Table 3.1 Main Stages in an Autotrophic Microalgal Biodiesel Life Cycle and the Energetic
        Aspects of Inputs and Co-Outputs






















































        Stages 1–6: cradle-to-gate; stages 1–7: cradle-to-pump; all stages: cradle-to-wheel.

        There is also no consensus on the energetic desirability of lipid-based autotrophic microalgal
        biofuels. Whereas some hold that energetic inputs in commercial autotrophic microalgal
        biofuel production will be much smaller than the energetic output embodied in those
        biofuels, 12,13,28  others hold that energetic outputs of microalgal biofuel production are and/or

        will be lower than, or not much exceed, inputs.      22,27  There is also disagreement about
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