Page 260 - Advances in Biomechanics and Tissue Regeneration

13.3 ELECTRIC AND ELECTROMECHANICAL CLUES 257

TABLE 13.1 Cells, Applied Stimuli (Electrical and/or Mechanical) and Material Used for Specific Representative Biomedical
Applications
Stimuli
Tissue Cells Electric Mechanical Material Reference
Cartilage Mesenchymal stem cell MSC 1Hz with Poly(vinylidene fluoride-trifluoroethylene) [69]
chondrogenic 10% deformation (PVDF-TrFE) and polycaprolactone (PCL)
fibers
Adipose-derived stem cells 20min application – [70]
(ADSCs) of 1kHz,
20mV/cm
Cardiac Human cardiac adipose tissue- 2ms pulses of 10% stretching Fibrin hydrogel [71]
derived progenitor cells 50mV/cm
(cardiac ATDPCs) at 1Hz
Human embryonic stem cells 1V/mm at 1Hz – [72]
(hESC, line H13) (1 and 90s)
Cardiomyocites of neonatal rat Biphasic 5% stretch Fibrin gel [73]
cardiac cells rectangular with 50% duty cycle
pulses, at 1Hz
1ms, 1Hz, 3V/cm
Cardiomyocytes biphasic pulses, Collagen/fibrin [74]
5V/cm, 0.2Hz, 3-D matrices
1ms
Cardiac adipose tissue-derived Monophasic Silicone-patterned surface [75]
progenitor cells (cardiac square-wave
ATDPCs) pulses,
2ms, 50mV/cm,
1Hz
Skin Human cutaneous fibroblasts 0.05V/mm (DC) Polypyrrole/poly(L-lactic acid) (PPy/ [76]
PLLA) membranes
Human skin fibroblasts 100mV/mm (DC) PPy/PLLA membranes [77]
Human dermal fibroblasts 100mV (DC) PPy-PDLLA membranes [78]
Muscle Satellite cells 2Hz In vivo [79]
Vascular smooth muscle cells 50μA (AC) PPy substrates [80]
0.05, 5, and 500Hz
Skeletal: the murine-derived 20V, 50ms pulse, Polydimethylsiloxane (PDMS) [81]
muscle cell line C2C12 1Hz micropatterned substrates
Muscle precursor cells (MPC) 70mV/cm, Micropatterned PLLA membranes [82]
33mHz
Mouse C2C12 myoblast cells 5V, 10ms, 1Hz Graphene oxide/polyacrylamide (GO/ [83]
PAAM) composite hydrogels
C2C12 Intermittent EF PCL aligned topography [84]
cycles
10min separated
by 20min for 3h
Nerve Rat PC-12 0.1V, 10 uA PPy film as anode [85]
Adipose-derived stem cells 1.5mA – [86]
toward the neuronal lineage
(C17.2)
Mouse neuronal cerebellum 100mV (DC) Polyaniline (PANI) with PCL/gelatin [87]
stem cells nanofibers
NIH-3T3 fibroblasts 0–200m (DC) PANI/poly(L-lactide-co-ε-caprolactone) [88]
(PLCL) nanofibers
Continued

II. MECHANOBIOLOGY AND TISSUE REGENERATION