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Advances in bio-oil extraction from nonedible oil seeds and algal biomass  191

           7.3.1 Seed preperation

           Seeds are prepared for oil extraction by initially removing the outer layer of the fruit to
           expose the kernel or seed, followed by drying of the kernel to the desired moisture con-
           tent [29]. Further, seeds are separated from fruits and the separated seeds are sieved,
           cleaned, andstoredatroomtemperature.Theseedsthatdonotdehiscearemechanically
           cracked open. The seeds and kernels that are separated from various sources are dried
           under the sun or in an oven at 30–0°C for 3–5days [30, 31].


           7.3.2 Kernel or seed extraction
           The first step to prepare seeds for oil extraction is to remove the outer layer of the fruits
           to expose the kernel. To obtain the kernel for further processing, the seeds are cracked
           open by using stompers or mallets. Because this step is the most time consuming,
           the seeds can be cracked open in a bulk amount by using stompers and mallets in bulk
           at a time [29].


           7.3.3 Kernel or seed drying

           The kernel or seeds are prepared in such a way that they contain the optimum moisture
           content for high oil extraction. It has been observed that the kernel with around 15%
           moisture content yields high oil by both mechanical and chemical extraction
           methods [29]. Seeds have to be dried before they undergo the oil extraction procees,
           and the process of seed drying is done carefully in a sieve tray drier by checking the
           moisture content of the seed several times in a day so as to remove at the desired
           dryness. The seeds are dried until the dry weights remain constant [29].


           7.4   Extraction techniques

           There are three main techniques that are available for the extraction of oil from a seed
           or kernel: mechanical, chemical, or enzymatic. Apart from these widely used
           methods, there are some other methods that are available that have been designed spe-
           cifically for some sample types: accelerated solvent extraction (ASE), SFE, or
           microwave-assisted extraction (MAE) [30].


           7.4.1 Mechanical extraction
           Oil extraction by mechanical expellers or presses is the most conventional method. In
           this method, either a manual ram press or an engine-driven screw press is used for the
           extraction of oil. It has been observed that the ram press can extract around 60%–65%
           of oil while an engine-driven screw press can extract around 68%–80% of the avail-
           able oil from seeds [30].
              The design of the mechanical press is very important as it plays a crucial role in the
           yield of oil. The yield can be increased by the pretreatment of the seeds by a cooking
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