Page 232 - Advances in Eco-Fuels for a Sustainable Environment

196 Advances in Eco-Fuels for a Sustainable Environment

Table 7.1 Different type of extraction techniques and their parameters

Mass of %of
dried Type of Extraction yield
Type of biomass extraction time, lipid/oil
microalgae/seeds (g) method temperature extracted Reference
Chlorella – Bligh and – 52.5 Widjaja
vulgaris dyer et al. [48]
Botryococcus – Solvent – 12.1 Tran et al.
braunii [49]
Scenedesmus – Wet milling – 25.3 Shen et al.
dimorphus [50]
Chlorella – Bead-beater – 18.8 Shen et al.
protothecoides Sonnication 10.7 [50]
Soxhlet 5.6
Crypthecodinium – SC-CO 2 – 8.6 Cravotto
cohnii et al. [51]
Nannochloropsis – SC-CO 2 – 25 Andrich
sp. et al. [52]
Spirulina – SC-CO 2 – 77.9 Andrich
(arthrospira) et al. [53]
platensis
Chlorococcum sp. – SC-CO 2 – 81.7 Halim
et al. [27]
Chlorella – Chloroform: – 5 Lee et al.
vulgaris methanol, [23]
1:1
Chlorella – n-Hexane – 25 Shen et al.
protothecoides [50]
Scenedesmus sp. – n-Hexane – 0.8 Ranjan
et al. [54]
Chlorella sp. 0.1 Soxhlet with –
chloroform
and
methanol
Isochrysis 5 Chloroform 60min, 25°C –
galbana and
methanol

7.6.2 Harvesting method
7.6.2.1 Centrifugation

Recovering microalgal biomass using centrifugation is carried out by appling centrif-
ugal force to the dilute suspension. Both increased biomass concetration and improved
harvest efficiency can be achieved within a very short time through this method. This
method is particularly adapted for high-value products such as food and therapeutic