Page 153 - Advances in Forensic Applications of Mass Spectrometry - Jehuda Yinon

1522_C03.fm Page 136 Thursday, November 13, 2003 9:53 AM

Abundance Scan 404 (12.456 min): 9501001.D
576
110000
100000
90000
80000
70000
60000
50000
40000
30000
20000 393
578
10000 178 197 235 239 368
102 1227 160 268 285 313 351 414 433 460 481 505 522 540
0
m/z 100 150 200 250 300 350 400 450 500 550
Figure 3.2 Mass spectrum of an HFB-derivative of stanozolol in the NCI mode,
with methane. (Reprinted from Cirimele, V., Kintz, P., and Ludes, B., J. Chro-
matogr. B, 740, 265, 2000. Copyright 2000, with permission from Elsevier Science.)

Ion 576.00 (575.70 to 576.70): 9201002.D
Abundance 12044
7000
6500
Stanozolol: 15 pg/mg
6000
5500
5000
4500
4000
3500
3000

Time
11.20 11.40 11.60 11.80 12.00 12.20 12.40 12.60 12.80
Figure 3.3 SIM mass chromatogram of a hair specimen of a bodybuilder, reveal-
ing the presence of stanozolol. (Reprinted from Cirimele, V., Kintz, P., and Ludes,
B., J. Chromatogr. B, 740, 265, 2000. Copyright 2000, with permission from
Elsevier Science.)
of hair material were used. The analysis of a strand of hair obtained from
two bodybuilders revealed the presence of methenolone at the concentration
of 7.3 and 8.8 pg/mg. Figure 3.4 shows the MS/MS-single reaction monitoring
(SRM) mass chromatograms of a 100-mg hair specimen of one of the body-
builders, revealing the presence of methenolone.

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