Page 247 - Algae

230 Algae: Anatomy, Biochemistry, and Biotechnology

TABLE 6.12
ASN-III Medium Composition

Reagents Per Liter Seawater
NaCl 25 g
MgCl 2* 6H 2 O 2 g
MgSO 4* 7H 2 O 3.5 g
0.75 g
NaNO 3
K 2 HPO 4* 3H 2 O 0.02 g
KCl 0.5 g
CaCl 2* 2H 2 O 0.5 g
Citric acid (C 6 H 8 O 7 ) 0.003 g
Ammonium ferric citrate (C 6 H 8 O 7 * nFe * nNH 3 ) 0.003 g
EDTANa 2 Mg 0.0005 g
0.02 g
Na 2 CO 3
Microelement stock solution 1 ml
Microelement Stock Solution
H 3 BO 3 2.860 g
MnCl 2* 4H 2 O 1.810 g
ZnSO 4* 7H 2 O 0.222 g
Na 2 MoO 4* 2H 2 O 0.390 g
CuSO 4* 5H 2 O 0.079 g
Co(NO 3 ) 2* 6H 2 O 0.0494 g
pH ¼ 7.5

TABLE 6.13
CHU-11 Medium Composition

Reagents Per Liter Seawater
MgSO 4* 7H 2 O 0.075 g
CaCl 2* 2H 2 O 0.036 g
1.5 g
NaNO 3
K 2 HPO 4* 3H 2 O 0.04 g
0.02 g
Na 2 CO 3
Na 2 SiO 3* 9H 2 O 0.58 g
Citric acid (C 6 H 8 O 7 ) 0.006 g
Ammonium ferric citrate (C 6 H 8 O 7 * nFe * nNH 3 ) 0.006 g
EDTANa 2 Mg 0.001 g
Microelement stock solution 1 ml
Microelement Stock Solution
H 3 BO 3 0.5 g
MnCl 2* 4H 2 O 2 g
Zn(NO 3 ) 2* 6H 2 O 0.5 g
Na 2 MoO 4* 2H 2 O 0.025 g
CuCl 2* 2H 2 O 0.025 g
Co(NO 3 ) 2* 6H 2 O 0.025 g
VOSO 4* 6H 2 O 0.025 g
HCl 1 M 3ml
pH ¼ 7.5