Page 249 - Algae

232 Algae: Anatomy, Biochemistry, and Biotechnology

TABLE 6.16
K Medium Composition

Reagents Per Liter Seawater
75 mg
NaNO 3
Na 2 -glycerophosphate * 5H 2 O 2.16 mg
NH 4 Cl 2.67 mg
Tris-base (pH 7.2) 121.1 mg
Microelement stock solution 1 ml
Vitamin solution 1 ml
Microelement Stock Solution
Fe Na EDTA 4.3 g
Na 2 EDTA * 2H 2 O 37.22 g
CuSO 4 * 5H 2 O 4.9 mg
MnCl 2 * 4H 2 O 178.2 mg
ZnSO 4 * 7H 2 O 22 mg
Na 2 MoO 4 * 2H 2 O 6.3 g
1.29 mg
H 2 SeO 3
FeCl 3 * 6H 2 O 3,15 g
CoCl 2 * 6H 2 O 11.9 mg
Vitamin Solution
Biotin (Vitamin H) 0.1 mg
Thiamine HCl (Vitamin B 1 ) 200 mg
Cyanocobalamin (Vitamin B 12 ) 1 mg
pH ¼ 7.2