Page 175 - Analytical method for food addtives
P. 175

19
                                                          14
                                                                     Dialysis of suspension. Conversion of nitrate to nitrite.
                                                              dihydrochloride. Red compound produced is measured
                                                                at 525 nm. Enzymatic reduction method: A measured
                                                            sulphanilamide and N-1(-naphthyl)ethylenediamine
                                                          Cd Column method: aqueous extract is treated with
                                                                  at 540 nm after addition of colour solutions
                                                                means of enzymatic reduction with nitrate
                                                          Dispersed in warm water, fat and proteins
                                                              through a Cd Reduction column or by
                                                                     Suspension in water. Transfer part of
                   Sulphanilic acid and N-1(-naphthyl)-  9 After 30 min the solution was passed through a sodium dodecyl sulphate coated alumina column and eluted with ethylenediamine solutions were added to 7 mL HCl–CH 3 COOH (2:3). A of effluent measured at form the azo dye  553 nm  10 Aqueous extract is treated with sulphanilamide and Homogenised,
                                                            precipitated, filtered. Either treated
                                                                  reductase
                              filtered
                                               Meat and fish  Turkish sucuk  (fermented  Milk and milk


                   Water    Meat    Beer         products     sausage)  products






                   Spectro-  photometric  Spectrometric  Enzymatic  spectrometric  Enzymic spectro-  photometric  Enzymic spectro-  photometric and  spectrometric  Segmented flow  analyses
   170   171   172   173   174   175   176   177   178   179   180